cd68 antibody mca1957ga  (Bio-Rad)

Journal: Marine Drugs

Article Title: Echinochrome A Treatment Alleviates Fibrosis and Inflammation in Bleomycin-Induced Scleroderma

doi: 10.3390/md19050237

Figure Lengend Snippet: Effects of EchA treatment on the number of macrophages and inflammation in scleroderma. The BLM-induced scleroderma mice were treated with or without EchA for three weeks. The mock-treated (PBS) and scleroderma (BLM or BLM+EchA) skin specimens were stained with anti-CD68 antibody together with anti-NOS2 or anti-TLR2 ( A ), anti-Arginase-I or CD163 ( B ) antibodies. Nuclei were stained with DAPI and overlaid images are shown. Scale bar = 50 μm. The numbers of CD68 + macrophages ( C ), M1 type macrophages (( D ); CD68 + TLR2 + and CD68 + NOS2 + cells), M1 type macrophages (( E) ; CD68 + Arginse-1 + and CD68 + CD163 + cells) were counted under high-power field. Data represent the mean ± SD ( n = 4 per group). * p < 0.05.

Article Snippet: Anti-CD68 antibody (MCA1957GA) was purchased from AbD Serotec (Raleigh, NC, USA).

Techniques: Staining

Journal: Mucosal Immunology

Article Title: Irf5 deficiency in myeloid cells prevents necrotizing enterocolitis by inhibiting M1 macrophage polarization

doi: 10.1038/s41385-019-0169-x

Figure Lengend Snippet: IRF5 is induced in infiltrated macrophages in human NEC neonates. a Representative immunohistochemical staining for IRF5 in the intestines of control and NEC neonates. NEC neonates exhibited a significant elevation in IRF5 expression. b Representative immunofluorescence staining for IRF5 (green) and CD68 (red) in control and NEC intestines. NEC led a significantly increased number of CD68-positive cells in the intestines, and IRF5 was mostly localized in CD68-positive cells. ( n = 5 in the control group; n = 10 in the NEC group. Scale bar: 50 μm in ( a ) and 100 μm in ( b ). * P < 0.05)

Article Snippet: The murine intestinal tissues were stained with an anti-IRF5 antibody (1:200, ab181553) from Abcam, an anti-CD68 antibody (1:200, MCA1957GA) from BioRad, and an anti-iNOS antibody (1:200, ab15323) from Abcam.

Techniques: Immunohistochemical staining, Staining, Expressing, Immunofluorescence

Journal: Mucosal Immunology

Article Title: Irf5 deficiency in myeloid cells prevents necrotizing enterocolitis by inhibiting M1 macrophage polarization

doi: 10.1038/s41385-019-0169-x

Figure Lengend Snippet: IRF5 is induced in infiltrated macrophages in murine experimental NEC. a Representative HE staining of samples from breast-fed pups and pups with NEC. Histological injury grade was significantly increased in the NEC group. b Representative immunofluorescence staining for IRF5 (green) and CD68 (red) in the intestines of mouse pups. The pups with NEC exhibited significantly increased IRF5 expression compared to that in the breast-fed pups, and IRF5 was mostly localized in infiltrated CD68-positive cells in the intestines. c Western blotting analysis showed that IRF5 expression in the intestine was significantly induced in the NEC pups compared to the breast-fed pups. ( n = 5 in breast fed; n = 10 in NEC. Scale bar: 100 μm. * P < 0.05)

Article Snippet: The murine intestinal tissues were stained with an anti-IRF5 antibody (1:200, ab181553) from Abcam, an anti-CD68 antibody (1:200, MCA1957GA) from BioRad, and an anti-iNOS antibody (1:200, ab15323) from Abcam.

Techniques: Staining, Immunofluorescence, Expressing, Western Blot

Journal: Mucosal Immunology

Article Title: Irf5 deficiency in myeloid cells prevents necrotizing enterocolitis by inhibiting M1 macrophage polarization

doi: 10.1038/s41385-019-0169-x

Figure Lengend Snippet: Infiltrated macrophages are polarized into the M1 phenotype in experimental NEC. a Representative immunofluorescence staining for iNOS (green) and CD68 (red) in the intestine. The pups with NEC exhibited a significant increase in the number of iNOS + CD68 + cells in the intestines compared to that in breast-fed pups. b Quantitative PCR analysis of the mRNA expression of M1 and M2 macrophage-associated genes in the intestines. The mRNA expression of M1 macrophage-associated genes was significantly increased in NEC, while the mRNA expression of M2 macrophage-associated genes, except that of Retnla , was unchanged. ( n = 5 in the breast-fed group; n = 10 in the NEC group. * P < 0.05)

Article Snippet: The murine intestinal tissues were stained with an anti-IRF5 antibody (1:200, ab181553) from Abcam, an anti-CD68 antibody (1:200, MCA1957GA) from BioRad, and an anti-iNOS antibody (1:200, ab15323) from Abcam.

Techniques: Immunofluorescence, Staining, Real-time Polymerase Chain Reaction, Expressing

Journal: Mucosal Immunology

Article Title: Irf5 deficiency in myeloid cells prevents necrotizing enterocolitis by inhibiting M1 macrophage polarization

doi: 10.1038/s41385-019-0169-x

Figure Lengend Snippet: Irf5 ablation in myeloid cells suppresses M1 macrophage polarization in experimental NEC. a Representative immunofluorescence staining for iNOS (green) and CD68 (red) in the intestines of the indicated groups. The pups with NEC exhibited a significant increase in the number of iNOS + CD68 + cells in the intestines compared to that in the breast-fed pups, while Irf5 deletion in myeloid cells markedly blocked this effect. b Quantitative PCR analysis of the mRNA expression of M1 and M2 macrophage-associated genes in the intestines of the indicated groups. The myeloid-specific deficiency of Irf5 significantly inhibited the NEC-induced mRNA expression of M1 macrophage-associated genes in the Irf5 fl/fl pups. The mRNA expression of the M2 macrophage-associated genes, except that of Retnla , was unaltered among the groups. ( n = 5 in the breast-fed Irf5 fl/fl group; n = 5 in the breast-fed Irf5 ΔMΦ group; n = 13 in NEC Irf5 fl/fl group; n = 12 in the NEC Irf5 ΔMΦ group. Scale bar: 100 μm. * P < 0.05)

Article Snippet: The murine intestinal tissues were stained with an anti-IRF5 antibody (1:200, ab181553) from Abcam, an anti-CD68 antibody (1:200, MCA1957GA) from BioRad, and an anti-iNOS antibody (1:200, ab15323) from Abcam.

Techniques: Immunofluorescence, Staining, Real-time Polymerase Chain Reaction, Expressing

Journal: Human molecular genetics

Article Title: Restoration of the balanced alpha/beta-globin gene expression in beta654-thalassemia mice using combined RNAi and antisense RNA approach.

doi: 10.1093/hmg/ddm218

Figure Lengend Snippet: Figure 6. Histopathologic analysis of mouse hematopoietic tissues. The following tissues were examined at 6 months after birth. (A) Bone marrow (original magnification, 100). Left: wild type. the marrow shows various stages of normal erythroid maturation. Megakaryocytes as well as a significant numbers of maturing myeloid cells are present. Center: b654 heterozygote (Hbbth-4/Hbbþ). Misshapen erythroid cells and red cell fragments are present in the sinuses. Some of the immature erythroid cells have dysmorphic nuclei. Mature erythroid cells are decreased. Right: aiba-Hbbth-4/Hbbþ. The marrow shows a relative increase in erythroid elements; myeloid cells are also present. (B) Spleen (original magnification, 100). Left: wild type. The red pulp contains a mixture of erythroid and myeloid cells. The sinuses are open. Center: Hbbth-4/Hbbþ. The red pulp consists primarily of immature erythroid cells. Mitotic figures are easily identified. The immature erythroid cells often have abnormal nuclei. Haemosiderin is obviously in red pulp. Right: aiba-Hbbth-4/Hbbþ. It showed less expansion of the red pulp and were morphologically intermediate between those of the healthy and b654 control mice. There is almost no haemosiderin in red pulp. (C) Liver (original magnification, 100). Left: wild type. Splenomegaly, EMH is absent. Center: Hbbth-4/Hbbþ. Extensive EMH is present. Right: aiba-- Hbbth-4/Hbbþ. Moderate EMH is present between those of the healthy and b654 control mice. (D) Immunohistochemistry analysis of CD68 in mouse spleens (original magnification, 200). Left: wide type. Almost no macrophages are found. Center: Hbbth-4/Hbbþ. Lots of macrophages are present. Right: ai- ba-Hbbth-4/Hbbþ. Fewer macrophase are present.

Article Snippet: Immunohistochemistry analysis was also performed as previously described (26), using rat antimouse CD68 polyclonal antibody MCA1957GA (Serotec Ltd, UK).

Techniques: Control, Immunohistochemistry